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Hiremath, Lingayya
- Hyper Immune Response against T-2 Mycotoxin in Oryctolagus Cuniculus
Abstract Views :137 |
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Authors
S. Narendra Kumar
1,
Lingayya Hiremath
1,
Praveen Kumar Gupta
1,
Ajeet Kumar Srivastava
1,
Akanksha Jain
1,
Abhishek R. Hegde
1,
L. Hamsa
1
Affiliations
1 Department of Biotechnology, RV College of Engineering, Bangalore, IN
1 Department of Biotechnology, RV College of Engineering, Bangalore, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 1 (2019), Pagination: 237-240Abstract
Development of standardize protocol for the production of antibodies produced due to hyper immune response in Oryctolagus cuniculus (Rabbit). Rabbits were selected as the ideal model organism because they are easy to bleed subsequently and a better hyper immune response is observed without the death of the organism. The hyper immune response was created against T2 toxin which was diluted; a mixture was made with Freund’s adjuvant (complete and incomplete) and injected into two rabbits kept under observation. One booster dose was given on the 7th day. The injection was given at three different sites and the rabbits were kept for observation. The rabbits were bleeding on 7th, 14th, 21st and 28th day and serum was separated from blood. The serum collected was analyzed for antigen antibody reaction using ELISA. From these studies we have concluded that at 1:100 serum dilutions and 1:50 antigen dilution the antibody-antigen reaction is the highest. It can be further concluded that at the high binding affinity of the toxin to the immobilized antibody, which is evident by the high binding and the low dissociation constant values obtained. This hyper immune serum consists of polyclonal antibodies that can be used for the development of various kit based immunosensors.Keywords
Rabbit, T-2 Mycotoxin, Hyperimmune Response, Antibody and ELISA.
References
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- Immunization, immune sera and the production of antibodies WOLF D. KUHLMANN, M.D. Division of Radiooncology, Deutsches Krebs for schungszentrum, 69120 Heidelberg, Germany.June 2008
- A capacitive immunosensor for detection of cholera toxin Mahmoud Labiba,b, Martin Hedströma, Magdy Aminb, Bo Mattiassona, A Department of Biotechnology, Lund University, Box 124, 221 00 Lund, Sweden b Microbial Biotechnology Center, Faculty of Pharmacy, Cairo University, Kasr El-Aini, Box 115 62.
- Wan, Q.; Wu, G.; He, Q.; Tang, H.; Wang, Y., The toxicity of acute exposure to T-2 toxin evaluated by the metabonomics technique. Molecular bioSystems 2015,11 (3), 882-91
- Escrivá, L.; Font, G.; Manyes, L., In vivo toxicity studies of fusarium mycotoxins in the last decade: A review. Food and Chemical Toxicology 2015, 78 (1), 185-206.
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- Cortinovis, C.; Pizzo, F.; Spicer, L. J.; Caloni, F., Fusarium mycotoxins: effects on reproductive function in domestic animals-a review. Theriogenology 2013, 80(6), 557-64.
- Goossens, J.; De Bock, L.; Osselaere, A.; Verbrugghe, E.; Devreese, M.;Boussery, K.; Van Bocxlaer, J.; De Backer, P.; Croubels, S., The mycotoxin T-2 inhibits hepatic cytochrome P4503A activity in pigs. Food and Chemical Toxicology 2013, 57, 54-6.
- Hydroxycitric Acid (Hca)-A Potent Nutraceuticals
Abstract Views :476 |
PDF Views:0
Authors
Affiliations
1 Department of Biotechnology, R V College of Engineering, Bengaluru, IN
1 Department of Biotechnology, R V College of Engineering, Bengaluru, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 7 (2019), Pagination: 3163-3168Abstract
On a global scale, obesity has reached epidemic proportions and is a major contributor to the global burden of chronic disease and disability [1]. Currently, more than one billion adults worldwide are overweight and at least 300 million of them are clinically obese (WHO, 2018). Currently, there are many treatments available for obesity; one such method involves the use of anti-obese molecules. Compounds such as Orlistat, Lorcaserin, Sibutramine, Metformin and Hydroxycitric acid (HCA) are used as anti-obese molecules. Among these, Hydroxycitric acid is the preferred molecule since it shows high efficacy in controlling obesity, with minimal side effects. Several pharmaceutical companies extract HCA from the fruits of Garcinia cambogia, a native tree grown in south India. Due to geographical and seasonal limitation of Gracinia fruits, an alternative method of producing HCA is necessary.Keywords
Garcinia cambogia, Hydroxy Citric Acid (HCA), Nuetraceutical, Antiobesity, Orlistat, Lorcaserin, Sibutramine, Metformin.References
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